Zlecenie - Zakup odczynników biologicznych 14 11 2018 A Odczynniki...

logicznych służących do realizacji badań w projekcie pt. „Rozwój innowacyjnej immunoterapii nowotworów litych celującej w immunosupresyjne mikrośrodowisko guza”. Przedmiot zamówienia 1. Phorbol-12-myristate-13-acetate (PMA), 5mg
• CAS number:16561-29-8;
• purity ≥ 95%;
• appearance : amorphous film, physical state: solid;
• a reversible and highly potent protein kinase C (PKC) activator;
• melting point : 50-70° C;
• solubility: soluble in DMSO (25 mg/ml), 100%ethanol (25 mg/ml), acetone, ether, DMF, methanol, and chloroform. Insoluble in water, and aqueous buffers;
Ilość: 1 kod CPV: 33696300-8
2. Rabbit IgG, polyclonal - Isotype control, 100 µg
• does not react with any antigen and was not produced using an immunogen;
• storage buffer: pH: 7.40; preservative: 0.02% sodium azide; constituent: PBS;
• concentration: 100 µg at 1 mg/ml;
• purity: protein G purified;
• application: Flow cytometry; references and images available on producer’s website;
Ilość: 1 kod CPV: 24956000-0
3. Goat anti-rabbit IgG H&L, 500 µg
• goat polyclonal secondary antibody to rabbit IgG - H&L conjugated with Alexa Fluor 488 (Ex: 495nm, Em: 519nm);
• suitable for: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr;
• storage buffer: preservative: 0.02% sodium azide; constituents: 30% glycerol, 1% BSA, PBS;
• purity: Immunogen affinity purified;
• references and images available on producer’s website;
Ilość: 1 kod CPV: 24956000-0
4. Anti-SOCS3 antibody, 100 µg
• rabbit polyclonal to SOCS3;
• species reactivity: Mouse, Rat, Human;
• storage buffer: preservative: 0.02% sodium azide; constituents: 1% BSA, PBS, pH 7.4;
• concentration:100 µg at 1 mg/ml;
• purity: Immunogen affinity purified;
• references and images available on producer’s website;
Ilość: 1 kod CPV: 24956000-0
5. Mouse Granzyme B ELISA kit, 96 tests
• species reactivity: mouse;
• sample type: cell culture supernatant, serum, plasma;
• assay type: sandwich (quantitative);
• sensitivity at least 24.5 pg/ml;
• detection: colorometric, range: 188 pg/ml - 12000 pg/ml;
• pre-coated microplate (12 x 8 well strips);
Ilość: 1 kod CPV: 33141625-7
6. IFN gamma Mouse uncoated ELISA kit, with plates, 10x96 tests
• contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA);
• analytical sensitivity: 15 pg/ml;
• assay range: 15-2,000 pg/ml;
• sample type: supernatants, serum;
Ilość: 1 kod CPV: 33141625-7
7. Luminescent kinase activity assays, 100 ml
• luminescent signal is inversely proportional to the amount of kinase activity;
• for automated high-throughput screening (HTS);
• kit format: 100μM (is linear to 100μM ATP);
• is sufficient for 2,000 assays at 50µl/assay in 96-well plates or 10,000 assays at 10µl/assay in 384-well plates;
Ilość: 5 kod CPV: 33141625-7