Zlecenie 7147353 - Zakup odczynników biologicznych i materiałów...

zamówienia Celem zamówienia jest zakup odczynników biologicznych i materiałów laboratoryjnych służących do realizacji badań w projekcie pt. "Nowa generacja immunoterapii nowotworów oparta o aktywację odpowiedzi immunologicznej pacjentów". Przedmiot zamówienia 1. VHL/CUL2/ELOB/ELOC/RBX1 Complex
• Species: Human
• Host Species: HEK293
• Description: VHL/CUL2/ELOB/ELOC/RBX1 complex of human VHL a.a. 2-212(end) with N-terminal FLAG-tag and MW= 25 kDa, human CUL2, a.a. 2-745(end) with N-terminal FLAG-tag and MW= 88 kDa, human ELOB, a.a. 2-118 with N-terminal FLAG-tag and MW= 14 kDa, ELOC, a.a. 2-112(end) with N-terminal His-tag and MW= 13 kDa human Rbx1, a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, individually expressed in a HEK293 expression system
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
• Stability: at least 6 months at –80°C
• Application: Useful for the study of protein degradation and PROTAC design, and selectivity profiling
Wielkość opakowania: 50 µg, Ilość: 2, kod CPV: 24956000-0

2. Donor beads coated with glutathione
• can be used to capture GST-tagged proteins and biomolecules
• 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume
Wielkość opakowania: 1 mg, Ilość: 1, kod CPV: 33696500-0

3. Anti-FLAG Acceptor Beads
• conjugated to anti-FLAG antibody
• recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites
• can be used to capture FLAG-tagged proteins and peptides
• 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume
• No-wash steps, no separation steps
• detect strong or weak interactions, from pM to mM affinity
• measure very large protein or antibody complexes – spanning up to 200 nm or more
• multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
Wielkość opakowania: 250 µg, Ilość: 2, kod CPV: 33696500-0

4. 10 % precast polyacrylamide gel
• 15 well, well volume: 15 μl;
• for separation of polypeptides 15–200 kD;
• tris-glycine extended; stain-free;
• stain-free imaging of gels and blots in 1 min;
• can be used for denaturing electrophoresis with standard running buffer containing SDS;
• laemmli-like separation in the presence of SDS ;
• run time as short as 15 min;
• shelf life: 12 months;
Wielkość opakowania: 10 pieces, Ilość: 10, kod CPV: 33696500-0

5. Glicyne, CAS: 56-40-6
• Assay: ≥99%
• Form: powder
• for electrophoresis
• impurities: ≤0.01% Insoluble matter
Wielkość opakowania: 1 kg, Ilość: 2, kod CPV: 33696500-0

6. Pre-stained protein ladder
• mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting;
• supplied in a loading buffer for direct loading on gels; no need to boil;
• two reference bands—orange at 70kDa and green at 10 kDa;
Wielkość opakowania: 10 x 250 µL, Ilość: 3, kod CPV: 24956000-0