Zlecenie 7021860 - Zakup odczynników biologicznych i materiałów...

zamówienia Celem zamówienia jest zakup odczynników biologicznych i materiałów laboratoryjnych służących do realizacji badań w projekcie pt. "Nowa generacja immunoterapii nowotworów oparta o aktywację odpowiedzi immunologicznej pacjentów". Przedmiot zamówienia 1. Formula for removing bound primary and secondary antibodies from membranes
• Form: Liquid
• Ready and easy to use
• For use with antibodies that are difficult to remove from western blots
• For use on nitrocellulose and PVDF membranes, whether still wet or already dry;
• No dilution necessary;
• Odor free;
• Time of incubation: 5-15 minutes at RT or 37°C for high affinity antibodies
Wielkość opakowania: 500 mL, Ilość: 1, kod CPV: 33696500-0

2. TMB-Blotting Solution
• protein bands visible in less than one minute
• protein bands stable after membrane drying
• no special equipment needed for visualization; produces dark bands
• no organic solvents are required to dissolve; no dilution necessary for use
• detection method: colorimetric
• substrate type: HRP (Horseradish Peroxidase) substrate
• contains soluble TMB (3,3',5,5'-tetramethylbenzidine)
• Applications: Chromogenic Western blot detection, Nitrocellulose membrane blots, PVDF membrane blots
Wielkość opakowania: 250 mL, Ilość: 1, kod CPV: 33696500-0

3. Heparin HP columns
• Suitable for purification of antithrombin III, coagulation factors, and other plasma proteins
• Chromatography technique: Group specific affinity
• Bed height: 25 mm
• Bed volume: 5
• Column i.d.”: 16 mm
• Flow rate: < 20 ml/min
• Pressure max. (over the packed bed during operation): 5 bar [0.5 MPa] (70 psi)
Wielkość opakowania: 5 x 5mL, Ilość: 1, kod CPV: 24950000-8

4. Thrombin Protease, 500 units
• Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
• Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
• Formulation: Solid easily soluble in cold and hot water
• Color: white
• Cleavage buffer: PBS: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3
• Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0\
• Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Wielkość opakowania: 1 sztuka, Ilość: 1, kod CPV: 24956000-0

5. Ovalbumin (257-264) chicken peptide
• assay ≥97% (HPLC);
• peptide from ovalbumin: SIINFEKL
• dedicated for T cell activation in immunology studies;
• amino acid sequence: Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu;
• lyophilized from 0.1% TFA in H2O;
• color: white to off-white
Wielkość opakowania: 1 mg, Ilość: 1, kod CPV: 24956000-0

6. Methanol, CAS: 67-56-1
• Purity (GC): ≥ 99.9 %
• For analysis
• suitable for a broad spectrum of classical lab applications
• Ethanol (GC): ≤ 0.05 %
• Acetone: ≤ 0.001 %
• Acetaldehyde: ≤ 0.001 %
• Formaldehyde: ≤ 0.001 %
• Chloride (Cl): ≤ 0.5 ppm
• Sulfate (SO₄): ≤ 1 ppm
• in glass bottle
Wielkość opakowania: 1 litr, Ilość: 6, kod CPV: 33696500-0